Alignment

Figure 2.14. Alignment Component Layout

Alignment Component Layout

The Alignment view is very good for browing large alignments, even if you don't have any expression data for them. There is a utility, "aln2cdt.pl" available in the helper-scripts package from website to facilitate the viewing of clustalw alignments.

The alignment view relies up the existence of a column named "ALN" containing all the sequence data, with IUPAC symbols for the amino acids, and non-symbol spacer characters such as dashes designating the gaps. The alignment view will render the matching sequence into a dendrogram-view like two-level display, complete with gene tree.

There are two PERL scripts in the helper-scripts package available from the website which should aid in the usage of alignment view, aln2cdt.pl which will create a cdt with the appropriate columns, and potentially a gtr fie if there is a dnd file available, and appendPCL.pl, which will allow you to append expression data in the pcl format to the cdt alignment file.

For a detailed howto to assist in making your own alignments, please see the fgf receptor example on the website (http://jtreeview.sourceforge.net)